RNA sequencing
Measure which genes are switched on, and by how much, under different conditions. RNA sequencing is used to study stress responses, infection, development and disease.
- Turnaround
- TAT working days
- Price
- From BDT price per sample
- Platforms
- Illumina
What we offer
- Transcriptomics (RNA-Seq)
- mRNA-Seq
- Gene expression profiling
- mRNA analysis
- Small RNA analysis
Who it is for
Researchers comparing gene expression between treatments, tissues, strains or time points.
What you receive
- Raw data (FASTQ)
- Quality-control report
- Differential expression analysis on request confirm
Sample requirements
General requirements for sequencing. Client: add any requirement specific to rna sequencing
| Sample | Amount | Volume | Concentration | Purity |
|---|---|---|---|---|
| Total DNA | ≥ 200 ng | ≥ 20 µL | ≥ 10 ng/µL | A260/280 1.8–2.0 |
| cDNA | ≥ 200 ng | ≥ 20 µL | ≥ 10 ng/µL | A260/280 1.8–2.0 |
| PCR products | confirm | ≥ 20 µL | confirm | confirm |
| Total RNA | confirm | confirm | confirm | RIN ≥ ? / A260/280 |
Not sure if your sample qualifies? Message us before shipping. Full packing and shipping steps are on How to order.
Frequently asked questions
How should I preserve RNA samples?
Client: storage and shipping conditions for RNA, e.g. RNAlater, dry ice
How many replicates do I need?
At least three biological replicates per condition are recommended for reliable results.
Can you do the bioinformatics?
Yes, analysis support is available. Client: confirm standard RNA-Seq analysis package
Other services
Gene-specific sequencing
- Bacterial identification (16S rRNA)
- Fungus identification (ITS)
- Animal or fish identification (DNA barcoding)
- Identification of pure bacterial / fungal isolates
Whole genome sequencing
- Bacterial whole genome sequencing
- Bacteriophage whole genome sequencing
- Viral whole genome sequencing
- Fungal whole genome sequencing
Metagenomics & microbiome
- Metagenomics sequencing (16S rRNA)
- 18S sequencing
- ITS sequencing
- Shotgun metagenomics
Ready to start your project?
Tell us your organism, sample type and what you want to find out. We will reply with the right service and a quote.